Does anyway have nonlinear least squares Mathcad files that can analyze multiple enzyme kinetic data sets simultaneously(for example initial velocity v vs substrate concentration S at no and two or more different inhibitor concentrations). I would like to fit all the data sets together and get a global fit to find best values of the kinetic constants, Km, Vm, and Kinhib and inhibition model (competetitve, uncompetitive, mixed inhibition? I have used Clelands enzyme kinetics programs (Fortran) for years but would like have my students use Mathcad to do nonlinear global fits.
Thanks.
Henry Jakubowski
College St. Benedict/St. John's University
37 S. College Ave.
St. Joseph, MN 56374
hjakubowski@csbsju.edu
Thanks.
Henry Jakubowski
College St. Benedict/St. John's University
37 S. College Ave.
St. Joseph, MN 56374
hjakubowski@csbsju.edu
